Cypridina Luciferin, also known as Vargula Luciferin or Vargulin
[Cypridina was first characterized by Dr Osamu and Akemi Shimomura from \"Umi Hotaru\" Japanese Sea Fireflies in 1966. These are bottom dwelling ostracods that emit a light stream into water when disturbed presumably to deter predation. Cypridina Luciferase was first cloned by Eric Thompson, and S. Nagata under Fred Tsuji in Japan 1989. Cypridina is a 555 amino acid, 62.171 KDa protein that oxidizes the luciferin to produce blue light at 462 nm Em Max. The advantage of using the Cypridna system: it does not cross react with Coelenterazine or Firefly d-Luciferin so it may be used in multiplex assays, brighter then Firefly Luciferase.]
| Compound ID: | Vargulin or Cypridina hilgendorfi Luciferin |
| IUPAC Name: | 2-[3-[2-[(2S)-butan-2-yl]-6-(1H-indol-3-yl)-3-oxo-7H-imidazo [2, 1-c]pyrazin-8-yl]propyl]guanidine |
| Molecular Formula: | C22H27N7O |
| MW: | 405.5 |
| Structure: | |
How To Use Cypridina
Certificate of Analysis MSDS HPLC NMR
The luciferin is shipped as filtered, lyophilized, batch controlled substratein 500 µg and 1 mg (2x 500 µg) and 10 mgs( 2 x5 mg) aliquot.
The advantages of lyophilized luciferins are:
- pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
- faster to dissolve due to its fine crystal structure
- consistent quality between aliquotes
Shipping: via FedEx or UPS at RT, overnight or 2nd delivery. Store at -20C or below.
