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主营:天然发光的海洋生物蛋白或海洋生物荧光
℡ 4000-520-616
℡ 4000-520-616
Nanolight/v-Coelenterazine/370-500/500 ug
产品编号:370-500
市  场 价:¥1100.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$55.00
品      牌: NanoLight
公      司:Nanolight Technologies
公司分类:
Nanolight/v-Coelenterazine/370-500/500 ug
商品介绍

V-Coelenterazine(V-CTZ)isasyntheticanalogueofCoelenterazinewithan additionalBenzylringThiscompoundhastheuniqueABIlitytoshifttheemissionspectraofwildtypRenillaLuciferase,RLuc8andGreenRenillaLuciferasetolongerwavelengths.Pleaseseeour"HowToUse"fordetails.

 
CompoundID:
v-Coelenterazine(V-CTZ)orCoelenterazine-v
alternativename:
16-benzyl-5-hydroxy-13-[(4-hydroxyphenyl)methyl]-11,14,17-triazatetracyclo[8.7.0.0^{2,7}.0^{11,15}]heptadeca-1(10),2(7),3,5,8,13,15-heptaen-12-one
Structure:
C28H21N3O3
MW:
447.48
Solvent:
Werecommend NanoFuel-VSolvent, especiallydesignedforthiscompound.SolubilityinEthanolorMethanolisverylow.
 Structure:







 
References:













InouyeS,ShimomuraO.TheuseofRenillaluciferase,Oplophorus
luciferase, andapoaequorinasbioluminescentreporterproteinin
thepresenceofcoelenterazine analoguesassubstrate. Biochem
BiophysResCommun. 1997Apr17; 233(2):349-53. 
PMID:9144537

StepanyukGA,UnchJ,MalikovaNP,MarkovaSV,LeeJ,VysotskiES.
Coelenterazine-vligatedtoCa2+-triggeredcoelenterazine-binding
protein is astableandefficientsubstrateofthered-shiftedmutant
ofRenillamuelleri luciferase.AnalBioanalChem.
2010Oct;398(4):1809-17. 
PMID:20711766.

Dragulescu-AndrasiA,ChanCT,DeA,MassoudTF,GambhirSS.
Bioluminescenceresonanceenergytransfer(BRET)imagingof
protein-proteininteractionswithin deeptissuesoflivingsubjects.
ProcNatlAcadSciUSA.2011Jul19;108(29):12060-5.
PMID:21730157

ShiftinemissionwithRLuc8:











 
RenillaLuciferasesemissionspectra:












 
 
HowToUseV-Coelenterazine
 
CertificateofAnalysis    HPLC    NMR    MSDS
 
Theluciferinisshippedasfiltered,lyophilized,batchcontrolledsubstrate in500ugaliquots.
1mg=2x500ug. 10mgsarepackedasregularpowderinonetube.
Theadvantagesoflyophilizedluciferinsare:
  • pre-aliquotedinsmallamounts,freshsubstrateforeverysetofexperiments
  • longshelf-life(packedunderArgon)
  • fastertodissolveduetoitsfinecrystalstructure
  • consistentqualitybetweenaliquotes
 Shipping:viaFedExorUPSatRT,overnightor2nddelivery.Storeat-20Corbelow

 

品牌介绍

Reagent customization availableU

Prolume / NanoLight has over 20 years of experience in the field of bioluminescence. If available reagents/substrates do not match your experimental setup or packaging size, we are happy to customize them for you and help you find solutions. We are always grateful to work with Universities and Companies in order to expand the scientific horizon and solve problems.
Customized packaging solutions, especially for luciferase substrates, is key to prolonged  shelf-life and reproducible results. In order to address your needs please:

  • Call or contact us via the the form available at the bottom of the page
  • Detail your specific requirements
  • For special orders, minimum order quantity may apply

We look forward to helping illuminate your scientific endeavor.

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